Coding

Part:BBa_K1903007:Design

Designed by: Sofía Vieto Fonseca   Group: iGEM16_TEC-Costa_Rica   (2016-10-14)


IntC DnaE-Split TEV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 109
    Illegal SapI.rc site found at 419


Design Notes

This part is composed of a split Intein C terminal from Npu DnaE and the split TEV C terminal. These protein are expressed together and will separate from each other in the presence of the other split Intein N from Npu DnaE BBa_K1903006. Finally we also added the Double terminator BBa_B0015.

Source

This part was based on the following BioBricks: C terminal of the NpuDnaE K1362401 and C terminal half of the TEV BBa_K1159102.

References